Application

Research Sub CategoryTumor Markers

This Anti-ABCB5, clone 5H3C6 Antibody is validated for use in western blotting, immunofluorescence, flow cytometry, IHC & ELISA for the detection of ABCB5.

Research CategoryApoptosis & Cancer

Immunofluorescence Analysis: A 1:200-1:1,000 dilution from a representative lot detected ABCB5 in HepG2 cells.

Flow Cytometry Analysis: A 1:200-1:400 dilution from a representative lot detected ABCB5 in HepG2 cells.

Immunohistochemistry Analysis: A 1:200-1:1,000 dilution from a representative lot detected ABCB5 in liver and breast cancer tissues.

ELISA: A representative lot was used on Control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATP-binding cassette sub-family B member 5, also known as ABCB5 P-gp or P-glycoprotein ABCB5, and encoded by the gene name ABCB5, belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules. ABCB5 is responsible for the resistance to doxorubicin of a subset of malignant melanomas and is expressed in several malignant tissues.

Immunogen

Purified recombinant fragment of human ABCB5 expressed in E. Coli

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected ABCB5 in A431 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~90 kDa observed. Uncharacterized bands may be observed in some lysate(s).