Application

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Amino-terminal FLAG-BAP^™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.

General description

Amino-terminal FLAG-BAP^™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP^™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.

The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Control protein

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,0.05 mM ZnCl2 in 50% glycerol, pH 8.0.