Application

Acriflavine is used to fluorescently label high molecular weight RNA molecules .Excitation and emission wavelengths in various solvents : Methanol: λex = 424 nm; λem = 518 nm Ethanol: λex = 426 nm; λem = 524 nm Propanol: λex = 430 nm; λem = 512 nm Butanol: λex = 430 nm; λem = 526 nm Formamide: λex = 434 nm; λem = 524 nm Glycerol: λex = 432 nm; λem = 540 nm Water: λex = 416 nm; λem = 514 nmInsoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor .

Acriflavine has been used in the agglutination test to distinguish between smooth and rough colony formation of Brucella melitensis.It has been used as an Ago2 (argonaute 2) inhibitor.It has been used as an inhibitor of HIF-ARNT (hypoxia-inducible factor - aryl hydrocarbon receptor nuclear translocator) complex formation.It has been used to study the bacteriocin production by Carnobacterium piscicola.

Biochem/physiol Actions

Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.

Packaging

25, 100 g in glass bottle