Application

Auresine can be used for isolation of protoplasts, nucleic acids, proteins, lipids and other components of Gram-positive bacterial cells. Auresine is particularly useful to lyse MRSA.. Thanks to its unique activity in non-physiological conditions (low conductivity and low temperatures), isolated components after auresine treatment, like nucleic acids and proteins, are not prone to degradation by released cellular enzymes.

General description

Auresine is a portion of the zinc metalloprotease Staphylococcus aureus. The enzyme has glycyl-glycine endopeptidase activity and it specifically cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, including various strains of methicillin-resistant Staphylococcus aureus (MRSA). Auresine is thus useful for lysis of MRSA.

Preparation Note

Auresine in soluble in water (20 mg/ml), yielding a clear solution. The product is active in 100 nM concentration (1.4 ug/mL) in low conductivity buffers (= 2 mS/cm) at temp. 0-40°C.

Storage and Stability

Auresine lyophilized powder should be stored at room temperature. Once dissolved in storage buffer, Auresine is stable at room temperature up to 4 months, at 4°C, -20°C, -80°C at least 2 years. Storage buffer: 20 mM Tris-HCl, pH 7.0, 200 mM NaCl, 10% glycerol.

Unit Definition

One unit will reduce the turbidity (A600) of suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes in 50 mM glycine, pH 8.0 at 25°C in 6.0 mL reaction mixture.