Application

Western Blotting Analysis: A 1:2,500 dilution of this antibody detected IFIT3 in 40 µg of IFN beta treated RAW264.7 cell lysate (Courtesy of Dr. Ganes C. Sen, Cleveland Clinic, OH).
Western Blotting Analysis: A representative lot detected Ifit3/ISG49 induction in RAW264.7 and wild-type, but not IRF3 or Stat1 knockout, MEFs upon Sendai virus/SeV infection, dsRNA or INF-beta stimulation in cultures, as well as in liver/lung/spleen/colon tissues from mice after dsRNA or INF-alpha i.v. injection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Western Blotting Analysis: A representative lot detected differential Ifit3/ISG49 inductions between murine MPC podocytes and primary murine glomerular mesangial cells in response to Sendai virus (SeV), encephalomyocarditis virus (EMCV), and vesicular stomatitis virus (VSV) infection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Western Blotting Analysis: A representative lot selectively detected exogenously expressed murine Ifit3/ISG49 and human IFIT3/ISG60, but not murine Ifit1/ISG56 or Ifit2/ISG54, in transfected HT1080 cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Western Blotting Analysis: A representative lot detected Ifit3/ISG49 induction in IFN-alpha-stimulated primary murine spleen B cells and in IFN-beta-stimulated MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053; Fensterl, V., et al. (2012). PLoS Pathog. 8(5):e1002712).
Immunoprecipitation Analysis: A representative lot selectively immunoprecipitated exogenously expressed murine Ifit3/ISG49, but not Ifit1/ISG56, from transfected MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Immunohistochemistry Analysis: A representative lot detected differential Ifit3/ISG49 induction among various cell types in formaldehyde-fixed, paraffin-embedded kidney tissue sections from mice after Sendai virus (SeV) or dsRNA i.v. injection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Flow Cytometry Analysis: A representative lot detected IFN-beta-induced Ifit3/ISG49 immunoreactivity using murine bone marrow-derived dendritic cells, as well as isolated CD3+ (T cells) and B220+ (B cells) peripheral blood cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Immunocytochemistry Analysis: A representative lot detected IFN-beta-induced Ifit3/ISG49 expression in MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Flow Cytometry Analysis: A representative lot (1:500 dilution) detected IFN-beta-induced Ifit3/ISG49 immunoreactivity in primary murine macrophages (Courtesy of Dr. Ganes C. Sen, Cleveland Clinic, OH).

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

This Anti-IFIT3 Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry, Immunocytochemistry for the detection of IFIT3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interferon-induced protein with tetratricopeptide repeats 3 (UniProt Q64345; also known as GARG-49, Glucocorticoid-attenuated response gene 49 protein. IFIT-3, IRG2, p49) is encoded by the Ifit3 (also known as Garg49, Ifi49, Isg49) gene (Gene ID 5959) in murine species. IFIT1, IFIT2, and IFIT3 constitute the family of interferon-stimulated genes (ISGs) that are induced in response to virus infection, stimulation by interferons (IFNs), or double-stranded RNA (dsRNA). The proteins encoded by IFIT1 and IFIT2 are called ISG56 (p56) and ISG54 (p54) in both human and murine species, while the protein encoded by IFIT3 is of a different size in murine (ISG49 or p49) than its human counterpart (ISG60 or p60). Studies conducted in murine species reveals that the three members exhibit distinct properties and induction patterns in different cell types. While all three murine proteins are shown to bind the c-subunit of the translation initiation factor eIF3, p49/Ifit3 does not inhibit protein synthesis as p56/Ifit1 and p54/Ifit2.

Immunogen

His-tagged recombinant protein corresponding to mouse IFIT3.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in IFN beta treated RAW264.7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IFIT3 in 10 µg of IFN beta treated RAW264.7 cell lysate.

Specificity

Reacts with murine Ifit3/ISG49 and human IFIT3/ISG60, but not murine Ifit1/ISG56 or Ifit2/ISG54.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~48 kDa observed