Application
Detection of ACE1 activity in Tissue/Cell lysates, Serum, Plasma Enables to screen for ACE inhibitors
Features and Benefits
Our economical ACE Kit also saves your time and efforts: A simple, quick, sensitive, and direct procedure for measuring ACE levels in a variety of samples such as serum and plasma Enables to screen for ACE inhibitors Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data! User friendly: No need to weigh or mix multiple reagents Quick instruction bench card – to assure your experimental success More experiments in one kit - contains sufficient reagents for 200 tests.
General description
The Angiotensin I Converting Enzyme (ACE) Activity Assay is based on the cleavage of a synthetic fluorogenic peptide. The measured fluorescence is directly proportional to the ACE activity present. The reaction is summarized as follows: ACE
ACE Fluorogenic substrate ———————> Cleaved ACE Substrate + fluorescent product (λ
ex 320nm/λ
em 405nm) The ACE Positive Control in the kit can be used to screen for ACE inhibitors. This kit has a linear range of 1.5-110 mU of ACE activity. Angiotensin Converting Enzyme (ACE) is a key component in the renin-angiotensin system (RAS), which is well known for its role in the regulation of blood pressure, electrolyte balance, and vascular remodeling
1,2 . ACE is located mainly in the capillaries of the lungs, but can also be found in endothelial and kidney epithelial cells
3. This hormone system also regulates body fluids volume. Renin produces angiotensin I by cleaving several amino acids from angiotensinogen. ACE then hydrolyzes angiotensin I to yield active angiotensin II. Angiotensin II also stimulates the adrenal cortex to secrete aldosterone, which also causes an increase in blood pressure by stimulating sodium reabsorption by the kidney
1. Since ACE exhibits critical functions regulating RAS, ACE inhibition has become a promising approach for drug targeting in the treatment of cardiovascular diseases such as hypertension, heart failure, and diabetic nephropathy.The detection range : 1.5-110 mU of ACE activity.
Unit Definition
One unit of ACE is defined as the amount of enzyme that releases 1 nmol of fluorescent product from the substrate, in 1 minute, under the assay conditions, at 37 °C.This kit contains sufficient reagents for 200 tests.
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