Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Kd = 7 nM against inducible nitric oxide synthase (iNOS)

Cell permeable: yes

Primary TargetiNOS

General description

A selective, irreversible, slow, tight binding inhibitor of inducible nitric oxide synthase (iNOS, K_d = 7 nM) both in vitro and in vivo. Has a protective effect against cerebral ischemia by reducing ischemic lesion volumes. Exhibits greater than 5000- and 200-fold potency against human iNOS relative to eNOS and nNOS, respectively, and greater than 1000-fold potency against rat iNOS relative to eNOS.

A selective, cell-permeable, irreversible, slow, tight-binding inhibitor of inducible nitric oxide synthase (iNOS; K_d = 7 nM). Suitable for use in both in vitro and in vivo systems. Has a protective effect against cerebral ischemia by reducing ischemic lesion volumes. Exhibits greater than 5000- and 200-fold potency against human iNOS relative to eNOS and nNOS, respectively, and greater than 1000-fold potency against rat iNOS relative to eNOS.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Parmentier, S., et al. 1999. Br. J. Pharmacol.127, 546.Cardenas, A., et al. 1998. Eur. J. Pharmacol. 354, 161.Parrino, P.E., et al. 1998. Ann. Thorac. Surg. 66, 733.Garvey, E.P., et al. 1997. J. Biol. Chem. 272, 4959.Laszlo, F., and Whittle, B.J. 1997. Eur. J. Pharmacol. 334, 99.Thomsen, L.L., et al. 1997. Cancer Res. 57, 3300.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)