Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST^™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST^™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.

SIGMAFAST^™ Fast Red TR/Naphthol AS-MX tablets has been used in fluorescentin situhybridization for the analysis of gene expression in neurons and synapses.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Reconstitution

Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.