Analysis Note

ControlJurkat cells.

Application

Research CategoryStem Cell Research

Detect Human-Nuclei using this Milli-Mark Anti-Human-Nuclei-PE Antibody, clone 235-1 validated for use in FC.

Research Sub CategoryNeural Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Stem-cell and fetal-cell replacement therapies for many diseases have proven to be very hopeful. Human nuclei antibodies are very important histological and cytological markers for identifying human cells. The use of human nuclei and C-peptide to force differentiation of human mesenchymal stem cells into insulin-producing cells to treat diabetes mellitus in mice has been successful. Human nuclei cells have also been associated with using stem-cell treatment in spinal cord injuries in canines.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Protein A purified

Quality

Evaluated by flow cytometry using Jurkat cells.

Specificity

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.