Analysis Note

ControlA-431 cell lysate

Application

Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~90 kDa. Weak reactivity is seen to dvl-1 and dvl-2 due to protein-protein interaction, and is not abolished following peptide pre-adsorption.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Anti-Dishevelled-3 Antibody detects level of Dishevelled-3 & has been published & validated for use in WB.

Research CategoryStem Cell Research

Research Sub CategoryHematopoietic Stem CellsNeural Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DVL3 is a member of multigene family that also contains DVL1 and DVL2. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL3 encodes a predicted 716 amino acid polypeptide that shows 74% nucleotide similarity with human DVL1. Similarity is particularly high in the N-terminal region and there is more divergence in the C-terminal regions.

Immunogen

Synthetic peptide corresponding to amino acids 582-596 of human and mouse disheveiled-3 protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Specificity

Disheveiled-3 protein (dvl-3). Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-3 protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots (not less than 10 mL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.