ANTI-SPI1

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

Spi-1 proto-oncogene (SPI1) mediates transcriptional regulation by DNA binding and protein interactions via its ETS domain. SPI1 is crucial for the fetal lymphoid and myeloid differentiation. PU.1 interaction with GFI-1 (growth factor independence-1) is essential for macrophage granulocyte development. High expression of Spi1 leads to senescence in erythroid progenitors and is regarded as critical step to target leukemia. It also interacts with microRNA, miR-424 and modulates macrophage differentiation. Deletions in the SPI1 gene is implicated in acute myeloid leukemia (AML). SPI1 gene inactivation results in fetal death.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. Protein array of 364 human recombinant protein fragments.

General description

Spi-1 proto-oncogene (SPI1), also called PU.1, belongs to E26 transformation-specific (ETS) transcription factor family and is highly expressed in hematopoietic stem cells. SPI1 gene is mapped to human chromosome 11p11.2. It has N-terminal transcriptional activation domain with acidic amino acid sub domains, a central proline, glutamic acid, serine, and threonine-rich (PEST) region and a C-terminal ETS domain.

Immunogen

Spi-1 proto-oncogene

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Corresponding Antigen APREST84738.

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.