Application

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected acetyl Histone H3 (Lys122) in HeLa and A431cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl Histone H3 (Lys122) in Histone peptides with various modifications.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated acetyl Histone H3 (Lys122) in 500 µg of sodium butyrate treated HeLa acid extract.

Anti-acetyl Histone H3 (Lys122) is an antibody against acetyl Histone H3 (Lys122) for use in WB, ICC, DB, IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

Immunogen

KLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys122) near the C-terminal.

Epitope: Near C-terminal

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected acetyl Histone H3 (Lys122) in 10 µg of sodium butyrate treated HeLa acid extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed