Anti-AMBRA1

Application

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected AMBRA1 in human brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected AMBRA1 in human brain cells.

Anti-AMBRA1 Antibody is an antibody against AMBRA1 for use in western blotting, IHC & immunofluorescence.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation (3), is in turn regulated by AMBRA1. AMBRA1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of AMBRA1 in cultured cells though RNA interference decreased the level of rapamycin and nutrient starvation-induced autophagy (4). Multiple isoforms of AMBRA1 are known to exist.

Immunogen

KLH-conjugated synthetic peptide corresponding to the N-terminus of human AMBRA1.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected AMBRA1 in rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 6 isoforms produced by alternative splicing ranging between ~84 kDa and ~143 kDa