Analysis Note

ControlMouse retina tissue lysate

Application

Research CategoryApoptosis & Cancer

Detect the Megf10 protein using this Anti-Megf10 validated for use in WB & IF.

Research Sub CategoryApoptosis - Additional

Immunofluorescence Analysis: A representative lot was used by an independent laboratory in P7 caudal brain tissue and retina cells. Image courtesy from the laboratory of Dr. Joshua Sanes, Harvard University.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Multiple epidermal growth factor-like domains protein 10 (Megf10) is a type 1 transmembrane protein with multiple EGF-like extracellular domains. Megf10 is highly expressed in the brain, and plays an important role in phacocytosis of apoptotic cells. This role may be performed through the interaction of Megf10 with the ATP-binding cassette transporter (ABCA1) protein. Previous studies have suggested that Megf10 may be involved in the uptake of amyloid β peptides in the brain. Megf10 may therefore play a role Alzheimer's disease.

Immunogen

Epitope: Cytoplasmic domain

Histidine-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Megf10.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse retina tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Megf10 on 10 µg of mouse retina tissue lysate.

Specificity

This antibody recognizes Megf10 at the cytoplasmic domain.

Other homologies: Rat (98% sequence homology) and Human (92% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed