General description

Full-length, recombinant, human, phosphodiesterdase 10A2 (accession no. 10A2) fused at the N-terminus to a His Tag^7reg; sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kotera, J., et. al. 2004. J. Biol. Chem.279, 4366.Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.

Packaging

10 u in Plastic ampoule

Physical form

In RIPA buffer (150 mM NaCl, 10 mM Tris-HCl, 1% NP-40), pH 8.3.

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µmol cAMP to AMP per min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)