Analysis Note

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Application

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using: ELISAImmunohistocytochemistryIn situ hybridizationWestern blot

Biochem/physiol Actions

In the presence of Na⁺, Mg²⁺ and ATP, digoxigenin inhibits sodium pumps.

General description

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.

Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: •ELISA: for coating: 2 to 4 μg/ml•Immunohistocytochemistry: 0.5 to 2 μg/ml•In situ hybridization: 0.2 to 0.4 μg/ml•Western blot: 0.5 to 2 μg/mlWorking solution: For coating applications: PBS (phosphate buffered saline), pH 7.4

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

Reconstitution

Add 1 ml PBS to a final concentration of 200 µg/ml.

Specificity

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.