Analysis Note

ControlA431 cell lysate, whole cell lysate from Jurkat cells

Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Detect IKKβ using this Anti-IKKβ Antibody validated for use in WB, IH(P).

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of IKKβ Polyclonal Antibody: HeLa Cells

Western blot: 1:500 to 1:1000 from a previous lot was tested on whole cell lysate from Jurkat cells can be used as positive control and an approximately 87 kDa band should be detected.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rabbit anti-IKKβ polyclonal antibody was raised against a peptide corresponding to amino acids 662 to 680 of human IKKβ (3,4), which differs from the corresponding murine sequence by one amino acid.

Linkage

Replaces: AB16522

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Purified rabbit polyclonal IgG in PBS containing 0.02% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on A431 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected IKK BETA on 10 µg of A431 lysates.

Specificity

This antibody recognizes IKKbeta, does not cross reacts with IKKα or IKKγ.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~87 kDa