Analysis Note

ControlCalyculin treated Jurkat cell lysate

Application

Detect Phosphothreonine-Proline using this Anti-Phosphothreonine-Proline Antibody validated for use in WB.

General description

Phosphothreonine-Proline, along with similar motifs involving serine/threonine residues preceding proline undergoing phosphorylation, has critical roles in the moderation of various cellular processes, protein function, and kinases through proline-directed phosphorylation. The deregulation of proline-directed phosphorylation is known to be a factor in diseases such as cancer and Alzheimer's neurodegeneration.

Immunogen

KLH-phosphothreonine-proline amide
(pT-P-NH2) peptide conjugates

Linkage

Replaces: AB3192

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in TBS with 0.05% sodium azide and 50% glycerol.

Quality

Evaluated by Western Blot in Calyculin treated Jurkat cell lysate.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated proteins on 10 µg of calyculin treated Jurkat lysates.

Specificity

Recognizes the phosphorylated phothreonine-proline motif, which is the site of phosphorylation of many proline-directed protein kinase such MAPK and cyclin-dependent protein kinases. Also recognizes the phosphoserine-proline motif to a similar degree.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.