Analysis Note

ControlC2C12 Cell Lysate, Hela cells, rat or mouse brain

Application

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategorySignaling

Immunoprecipitation: 12.5 µg/mL dilution of a previous lot was ued in immunoprecipitation on rat brain tissue; immunoprecipitations with tissue extracts other than brain may require higher antibody concentrations.
Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of GSK3β Polyclonal Antibody: HeLa Cells

This AntiI-GSK-3b Antibody, a.a. 335-349 is validated for use in WB, IP, IH(P) for the detection of AntiI-GSK-3b.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer′s disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.

Immunogen

Epitope: a.a. 335-349

Rat GSK3 beta synthetic peptide (aa. 335-349), conjugated to KLH. This sequence is 100% identical to human GSK3 beta.

Linkage

Replaces: AB8687

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified polyclonal IgG antibody is supplied in PBS, pH 7.2, 50% glycerol and 0.09% sodium azide.

Protein A affinity purified

Quality

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected GSK3 BETA on 10 µg of C2C12 lysates.

Specificity

This antibody detects a strong 47 kDa protein, corresponding to the expected molecular mass of GSK3 beta.

Storage and Stability

Stable at –20 °C for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2°-8°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

This antibody detects a strong 47 kDa protein.