Analysis Note

ControlPositive Control Included: Jurkat cell lysate (12-303)

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Detect TCF-1 with Anti-TCF-1 Antibody, clone 7H3 (Mouse Monoclonal Antibody), that has been shown to work in ICC, IHC & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with b catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor a enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with b catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).

Immunogen

Maltose binding protein (MBP) fusion protein corresponding to residues 102-269 of human TCF-1A splice variant. The epitope maps to a region just N-terminal to the High Mobility Group (HMG) box

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1159

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.5, 0.15M NaCl and 0.05% sodium azide

Quality

routinely evaluated by immunoblot on Jurkat RIPA cell lysate

Specificity

Recognizes multiple splice isoforms of TCF-1.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

26 kDa, 32 kDa, 38 kDa, 42 kDa, and 48 kDa