Application

Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected EAAT2 in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected EAAT2 in human and mouse Brain tissue lysates.Affinity Binding Assay: A representative lot of this antibody bound EAAT2 peptide with a KD of 5.8 x 10-7 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EAAT2 in Rat astrocytes.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected EAAT2 in human brain and rat brain tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-EAAT2, clone 1K8 ZooMAb, Cat. No. ZRB1630, is a Rabbit monoclonal antibody that specifically targets EAAT2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Excitatory amino acid transporter 2 (EAAT2).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1K8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects excitatory amino acid transporter 2 (EAAT2). It targets an epitope within 20 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Excitatory amino acid transporter 2 (UniProt: P43004; also known as Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2, EAAT-2) is encoded by the SLC1A2 (also known as EAAT2, GLT1) gene (Gene ID: 6506) in human. EAAT-2 is a multi-pass homotrimeric membrane protein serves as a major sodium-dependent, high-affinity transporter that moves glutamate back into the cell following neurotransmission. It is expressed predominantly in astroglial cells where it is responsible for about 90% of total glutamate uptake and maintains extracellular glutamate below excitotoxic levels. EAAT-2 functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. EAAT-2 contains 6 cytoplasmic domains, 5 extracellular domains, and 8 transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 1.5 to 1.8 nm, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move. Mutation in SLC1A2 gene can cause early infantile epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, K., et al. (2011). J. Cell Physiol. 226(10); 2484-2493).