PCTK3 (CDK18)/CYCLINY; ACTIVE

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Full-length recombinant human PCTK3 (CDK18) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 ⁽¹⁾. PCTK3 is expressed in a variety of human cell lines and tissues ⁽²⁾. Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer′s disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.