PI3K (P85 ALPHA) PROTEIN

Code: SRP0691-50UG D2-231

Application

Useful for Western blot and kinase assays.

Biochem/physiol Actions

Recombinant full-length human PI3K (p85alpha) was expressed by

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Your Price
€466.87 50UG
€574.25 inc. VAT

Application

Useful for Western blot and kinase assays.

Biochem/physiol Actions

Recombinant full-length human PI3K (p85alpha) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PI3K and platelet-derived growth factor receptor (1). Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) (2).

Reconstitution

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

accession no.NM_181523
assay70-95% (SDS-PAGE)
concentration0.2 µg/µL
formfrozen liquid
Gene Informationhuman ... PI3K (5295)
mol wt~86 kDa
recombinantexpressed in baculovirus infected Sf9 cells
shipped indry ice
storage temp.−70°C
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