GRANZYME B HUMAN

Biochem/physiol Actions

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

General description

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Physical form

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Unit Definition

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.