Application

Ganciclovir ready-made solution has been used in combination with resveratrol to study its effect on melanoma cells. It has also been used as a synthetic substrate for viral thymidine kinase to study its effects on human-inducible pluripotent stem cells.

Biochem/physiol Actions

Ganciclovir is a nucleoside guanosine analog that contains an active moiety, ganciclovir triphosphate. This active moiety can inhibit viral replication by interrupting the viral DNA during elongation. Ganciclovir is a potent agent in preventing cytomegalovirus (CMV) retinitis disease in human immunodeficiency virus (HIV) patients. It exhibits therapeutic effects against CMV disease involving the lungs, liver, and gastrointestinal tract. Ganciclovir can be enzymatically phosphorylated to an active triphosphate analog by herpes simplex virus thymidine kinase (HSV-TK) or host-encoded deoxyguanosine kinase. It has the potential to treat allogeneic bone marrow, kidney, heart, liver, and lung transplantations.

Other Notes

Ganciclovir ready made solution in DMSO is provided at 10mM concentration (2.55mg/mL). It is suggested to dilute the provided ready made solution to a working concentration of 2-100µM for cell culture applications.