Application

Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response.

Cell Line Origin

Skin

Components

Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO

General description

REK from Cell Applications, Inc. provide a suitable model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology. It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response (Nakai, 2008). Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons (Zhao, 2008). REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin (Jourdan, 2011).

Preparation Note

Primary culture, >500,000 cells in Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO These epithelial cells proliferate well when thawed and plated from cryopreservation, but they do not plate and proliferate after trypsinization

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Subculture Routine

Please refer to the RDF Culture Protocol.