Features and Benefits

Does not freeze in storage for quick, convenient use. Inhibits serine, cysteine, aspartic proteases and aminopeptidases. Tailored for use in tissue culture media. Suggested as 100 X solution. Reclosable screw-top vials. Can be stored in cooler for approx. 1 month or room temperature for a week.

General description

This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.