Features and Benefits

Does not freeze in storage for quick, convenient use. Tailored for use with Histidine fusion tagged proteins. Suggested as 100 X solution. Reclosable screw-top vials. Can be stored in cooler for approx. 1 month or room temperature for a week.

General description

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.