General description

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 6 mL of RPTEC Tox media supplement which is added to basal media and other components listed below to produce 500 mL of media needed for optimal culture conditions of Sigma’;s Human Renal Proximal Tubule Epithelial Cells (RPTEC) when used for Kidney Toxicology studies. The final RPTEC Tox media was developed specifically for use with Sigma’;s human RPTEC control SA7K Clone cells and various knockouts (KO).

Preparation Note

Prepare Tox Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-glutamine (G7513), 6 mL RPTEC Tox Supplement (MTOXRTSUP) and 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Tox Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO₂ in air atmosphere.