D-LACTATE ASSAY KIT

Code: MAK336-1KT D2-231

Features and Benefits

Compatible with high-throughput handling systems.

General description

Lactate is generated by lactate dehydrogenase (LDH) u...


read more

Your Price
€422.00 EACH
€519.06 inc. VAT

Features and Benefits

Compatible with high-throughput handling systems.

General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Principle

The D-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

Suitability

Suitable for D-lactate in serum, plasma, and cell media samples.

detection methodcolorimetric
storage temp.−20°C
usagesufficient for 100 colorimetric tests
This product has met the following criteria to qualify for the following awards:



PROCEED TO CHECKOUT

HAVE AN ACCOUNT? LOGIN


GUEST CHECKOUT

Proceed as a guest. You will have the option to register to access exclusive pricing and stock availability features after checkout.