Features and Benefits

Compatible with high-throughput handling systems.

General description

Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’;s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable.

Principle

The Glutamate Assay Kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample. Detection limit of 50 µM , linearity up to 2.5 mM glutamate in 96-well plate assay.

Suitability

Suitable for glutamate determination in serum, plasma, tissue extracts and food extract samples. Also suitable in drug discovery/pharmacology for studying the effects of drugs on glutamate levels.