General description

Triglycerides (TG) are the main constituents of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as an energy source. TG is broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.

Principle

The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In this assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a colorimetric (570 nm)/fluorometric (lex = 535 nm/lem = 587 nm) product. The kit is sensitive to detect 2 pmole–10 nmole (2–10,000 mM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

Suitability

Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.