General description

Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca²⁺, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity.

Principle

Calpain Activity Fluorometric Assay Kit is based on the cleavage of the calpain substrate, Ac-LLY-AFC. The Ac-LLY-AFC substrate emits blue light (max = 400 nm); upon cleavage by calpain, free AFC emits a yellow-green fluorescence ( max = 505 nm), which can be quantified using a fluorometer or a fluorescence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.

Suitability

Suitable for analyzing calpain activity in apoptotic and other samples.