Renin Assay Kit, sufficient for 100 fluorometric�tests

Application

Renin Assay Kit has been used to determine the activity of renin.

General description

Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension.

Principle

Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λ_ex = 540/λ_em = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.

Suitability

The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors.