Fluorometric Thiol Assay Kit, sufficient for 200 fluorometric�tests

Application

Fluorometric Thiol Assay Kit has been used to determine the lipid peroxidation expressed as thiols in samples.

General description

Thiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The disulfide bonds generated when the thiol groups of two cysteine residues are oxidized contribute to the tertiary or quatenary structure of a protein.

Principle

The Fluorometric Thiol Quantitation Kit provides an ultrasensitive assay to quantitate the thiol content of small molecules. The proprietary thiol sensor generates a strongly fluorescent adduct (λex = 490/λem = 520 nm) upon reacting with a thiol-containing compound. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM). The assay can be performed conveniently in either a 96 or 384 multiwell plate format.

Suitability

This kit is suitable for the detection of thiols in a variety of different biological samples including plasma, urine, and cell extracts. This kit is not optimized for measuring thiol groups in proteins.