Application
Sulfate Assay Kit has been used to determine the concentration of sulfate.
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
General description
Inorganic sulfate is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics, steroids, neurotransmitters, and bile acids. Sulfate is needed for the biosynthesis of cerebroside sulfate, glycosaminoglycans, and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human inherited osteochondro-dysplasia disorders. In mammals, sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules and only 5-20% of the filtered load is excreted into the urine.
Principle
The kit employs an improved method utilizes the quantitative formation of insoluble barium sulfate in polyethylene glycol. The turbidity measured at 600 nm is proportional to sulfate level in the sample.
Suitability
Suitable for the detection of sulfate in biological fluids such as serum and urine.
This product has met the following criteria to qualify for the following awards: