Anti-Myosin-3 (MYH3), clone BF-G6

Application

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Myosin-3 (MYH3) in 10 µg of rat embryonic muscle tissue lysate (Courtesy of Alberto Rossi, Ph.D., University of Colorado, U.S.A.).

ELISA Analysis: Clone BF-G6 hybridoma culture supernatant detected myosin from 10-week human fetus, but not myosin from 8-day new born muscle or adult skeletal muscle (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

Immunocytochemistry Analysis: Clone BF-G6 hybridoma culture supernatant immunostained aceton-fixed tumor cells in bone marrow aspiration from a child with rhobdomyosarcoma (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

Immunofluorescence Analysis: Clone BF-G6 hybridoma culture supernatant immunostained serial transverse cryosections of rat extraocular (EO) and soleus muscles. BF-G6 staining pattern is similar, but not identical to that of MYH15 (Rossi, A.C., et al. (2010). J. Physiol. 588(Pt 2):353-364).

Immunofluorescence Analysis: Clone BF-G6 hybridoma culture supernatant immunostained frozen fetal muscle sections, while very few cells were stained in 8-week new born muscle tissue and no staining of 8-month infant muscle tissue was observed (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

Immunohistochemistry Analysis: Clone BF-G6 hybridoma culture supernatant immunostained tumor cells in frozen human rhobdomyosarcoma sections (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

Western Blotting Analysis: Clone BF-G6 hybridoma culture supernatant detected myosin from 10-week human fetus, but not myosin from adult skeletal muscle (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

This mouse monoclonal Anti-Myosin-3 (MYH3) Antibody, clone BF-G6, Cat. No. MABT818 is validated for use in and Western Blotting, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blotting, for the detection of Myosin-3.

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myosin-3 (UniProt A6QPA6; also known as Muscle embryonic myosin heavy chain, Myosin heavy chain 3, MYH3, Protein, Myosin heavy chain, fast skeletal muscle, embryonic, SMHCE) is encoded by the MYH3 gene (Gene ID 281338) in bovine species. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. MYH3 is predominantly expressed during fetal development and its expression declines rapidly after birth. It is not normally present in adult muscle except during muscle regeneration. Mutations in MYH3 are known cause two congenital contracture syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome, the most severe forms of distal arthrogryposis (DA). Ref.: Pokrzywa, M., et al. (2015). PLoS One. 10(11):e0142094; Toydamir, R.M., et al. (2006). Nature Genet. 38(5):561-565.

Immunogen

Fetal bovine skeletal muscle myosin (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified.

Format: Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1κ.

Specificity

Clone BF-G6 detected myosin in human fetal muscle, but not adult human skeletal muscle tissue (Schiaffino, S., et al. (1986). Exp. Cell Res. 163(1):211-220).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~225 kDa observed. 223.7 kDa (bovine) and 223.9 kDa (human/rat) calculated. Uncharacterized bands may be observed in some lysate(s).