Anti-ZCCHC11 (TUT4), clone 9F6.1

Application

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: 4 µg/mL from a representative lot detected ZCCHC11 (TUT4) in 10 µg of C2C12 mouse myoblast and Jurkat human T-cell lysates.

Anti-ZCCHC11 (TUT4), clone 9F6.1, Cat. No. MABE1526, is a highly specific mouse monoclonal antibody that targets ZCCHC11 (TUT4) and has been tested in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Terminal uridylyltransferase 4 (EC 2.7.7.52; UniProt Q5TAX3; also known as TUTase 4, Zinc finger CCHC domain-containing protein 11) is encoded by the ZCCHC11 (also known as KIAA0191, TUT4) gene (Gene ID 23318) in human. TUTase 4 is an uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. It is involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets and also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, and miR-143 and miR-200c. It is reported to catalyze the 3′ uridylation of miR-26A that targets IL6 transcript to abrogate the silencing of IL6 transcript and promote cytokine expression. It may also participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB.

Immunogen

GST-tagged recombinant human ZCCHC11 (TUT4) N-terminal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified.

Format: Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected ZCCHC11 (TUT4) in 10 µg of PC3 human prostate cancer cell lysate.

Specificity

Clone 9F6.1 targets an epitope in the N-terminal region of ZCCHC11 (TUT4).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~185 kDa observed. 185.2/81.14 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).