Anti-SETBP1, clone 63

Application

Western Blotting Analysis: A 1:4 dilution of clone 63 hybridoma culture supernatant detected SETBP1 in 30-50 µg of REH human acute lymphoid leukemia cell lysate (Courtesy of Jesper Christensen, Ph.D. and Kristian Helin, Ph.D., University of Copenhagen, Denmark).

Anti-SETBP1, clone 63, Cat. No. MABE1128, is a highly specific mouse monoclonal antibody that targets SETBP1 and has been tested in Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SET-binding protein (UniProt Q9Y6X0; also known as SEB, SETBP1) is encoded by the SETBP1 (also known as KIAA0437, MRD29) gene (Gene ID 26040) in human. SETBP1 is shown to bind the SET nuclear oncogene involved in DNA replication. It is expressed in various tissues with low levels in myeloid and monocytic cells and in CD34+ cells, however, higher expression is observed in myeloid malignancies. Overexpression of SETBP1 promotes leukemogenesis by enhancing full-length SET protein and then impairing the phosphatase activity of the tumor suppressor PP2A through the formation of a SETBP1-SET-PP2A complex. Patients with SETBP1 overexpression have a significantly shorter overall survival, and poor prognosis, particularly in the elderly patients. Mutations in SETBP1 gene are associated with Schinzel-Giedion midface retraction syndrome, which is characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations. (Ref.: Cristobal, I., et al. (2010). Blood 115(3): 615-625).

Immunogen

GST-tagged recombinant human SETBP1 N-terminal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETBP1 in 10 µg of HeLa cell lysate.

Specificity

Clone 63 targets the N-terminal region of human and mouse SETBP1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~175 kDa observed. 175.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).