Application

Anti-SMARCA3 (HLTF), clone 5-1 , Cat. No. MABE1107, is a highly specific mouse monoclonal antibody that targets HTLF and has been tested in Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected predominantly nuclear HLTF (SMARCA3) immunoreactivity in human cervical cancer tissue section.

General description

Helicase-like transcription factor (UniProt Q14527; also known as DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, Sucrose non-fermenting protein 2-like 3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3) is encoded by the HLTF (also known as HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1) gene (Gene ID 6596) in human. Helicase-like transcription factor (115 kDa) is a SWI/SNF (mating-type switching/sucrose non-fermenting) protein that uses ATP hydrolysis to remodel chromatin in multiple cellular processes. It is shown to regulate transcription by binding to the SV40 enhancer and the promoters Serpin E1 and HIV-1. It is well expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. It was initially characterized for its DNA binding and transcriptional activity, however, later was shown to act as an E3 ubiquitin ligase and was implicated in post-replication DNA repair by polyubiquitination of proliferating cell nuclear antigen (PCNA). The inactivation of HIP116 is shown to induce an elevated level of chromosome breaks and fusions in mouse embryonic fibroblasts after treatment with alkylating agents. HIP116 inactivation in transgenic mice is reported to disrupt cell cycle at the G2/M phase.

Ref.: Arcolia, V et al. (2014). BMC Cancer 14, 492.

Immunogen

GST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Immunohistochemistry in human breast cancer tissue section.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected predominantly nuclear HLTF (SMARCA3) immunoreactivity in human breast cancer tissue section.

Specificity

Clone 5-1 detected predominantly nuclear HLTF (SMARCA3) immunoreactivity by immunohistochemistry staining. Clone 5-1 epitope lies within an N-terminal region present in both spliced isoforms of human HLTF reported by UniProt (Q14527).

Target description

113.9/99.92 kDa (human isoform 1/2) calculated.