Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p62 Sequestosome-1 in A431 and NIH/3T3 cells.

Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected p62 Sequestosome-1 in one million HeLa cells.

Detect p62 using this mouse monoclonal Anti-p62 (Sequestosome-1) antibody, clone 11C9.2, Alexa Fluor^® 488 Conjugate, Cat. No. MABC32-AF488, validated for use in Flow Cytometry and Immunocytochemistry.

General description

Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human. Sequestosome-1 is a multifunctional protein that binds ubiquitin and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling pathway. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF-kappaB in response to upstream signals. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.

Immunogen

GST-tagged recombinant fragment of 200 amino acids from the internal region of human p62 (Sequestosome-1).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal antibody IgM in PBS with 1.5% BSA and 0.05% sodium azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p62 (Sequestosome-1) in HeLa cells.

Specificity

Clone 11C9.2, Alexa Fluor^® 488 Conjugate detects p62 in HeLa cells.

Target description

47.7 kDa calculated.