Anti-CKAP4; clone 9C4.4

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Detect Cytoskeleton-associated protein 4 using this mouse monoclonal antibody, Anti-CKAP4 Antibody & clone 9C4.4 validated for use in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cytoskeleton-associated protein 4, also known as 63-kDa cytoskeleton-linking membrane protein or Climp-63 or p63, and encoded by the gene CKAP4, is an integral membrane protein that links the endoplasmic reticulum (ER) to microtubules. The binding of CKAP4 is dependent upon its phosphorylation state: dephosphorylated CKAP4 binds microtubules and phosphorylated CKAP4 does not bind microtubules. CKAP4 is localized to the endoplasmic reticulum membrane and plasma membrane; CKAP4 is also found in the cytoplasm and perinuclear regions upon APF stimulation. CKAP4 is expressed is a wide variety of cell types and tissues and CKAP4 appears to be critical for antibiotic cytotoxicity for certain drugs like Gentamycin which bind the drug and cause CKAP4 to dimerize and malfunction.

Immunogen

Epitope: coiled coil domain

GST-tagged recombinant protein corresponding to the coiled coil domain of human CKAP4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected CKAP4 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 65 kDa observed. Uncharacterized band(s) may appear in some lysates.