Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

Mucin family of proteins is involved in epithelial cell protection, regulation of adhesion and cell signaling. MUC3A (mucin 3A) confers protection to intestinal epithelial cells against the external environment. Variations in this gene determine the susceptibility of an individual to ulcerative colitis and Crohn′s disease (CD). This protein is expressed in synovial lining cells, macrophages, and fibroblasts, and it is overexpressed in synovial tissue. Therefore, it might play a role in the pathogenesis of arthritis. Hypomethylation of the 5′-flanking region of MUC3A gene determines the expression of this gene in various cancers such as breast, pancreas, colon and lung cancer cell lines. Therefore, methylation studies of this gene might help in predicting cancer risk as well prognosis of cancer patients. This gene is also up-regulated in renal clear cell carcinoma, and is involved in tumorigenesis of renal cancer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. Protein array of 364 human recombinant protein fragments.

General description

MUC3A (mucin 3A) belongs to the family of epithelial mucins, which are both secreted as well as cell surface glycoproteins. This family is expressed by epithelial tissues, and MUC3A is predominantly expressed in the small intestine. This gene maps to human chromosome 7q22. MUC3A contains an extracellular region with two cysteine-rich EGF (epidermal growth factor)-like domains, intervened by an N-glycosylation-rich domain, along with a putative coiled-coil domain. This is succeeded by a transmembrane domain, and a cytoplasmic region consisting of 75 amino acids. MUC3A has an alternatively spliced isoform which contains the first EGF- like domain, but lacks the second EGF-like domain and the transmembrane region.

Immunogen

Mucin-3B recombinant protein epitope signature tag (PrEST)

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Corresponding Antigen APREST70010.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide