Cell Line Description

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Components

HLA002A-1VL: Jurkat B2M KO CellsHLA002B-1VL: Jurkat HLA-A*02:01 CellsHLA002C-1VL: Jurkat HLA-A*01:01 CellsHLA002D-1VL: Jurkat HLA-A*03:01 CellsHLA002E-1VL: Jurkat HLA-A*11:01 CellsHLA002F-1VL: Jurkat HLA-A*24:02 CellsHLA002G-1VL: Jurkat HLA-B*15:10 CellsHLA002H-1VL: Jurkat HLA-B*07:02 CellsHLA002I-1VL: Jurkat HLA-B*08:01 CellsHLA002J-1VL: Jurkat HLA-B*35:01 CellsHLA002K-1VL: Jurkat HLA-B*40:01 Cells

Features and Benefits

Unlike wild type Jurkat cells, which express multiple HLA subtypes, the Jurkat HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens.

General description

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88042803.This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background.  The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.  CompoZr^® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS).  MISSION^® lentivirus was used to generate the ten (10)  monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis.  

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

MISSION Use Licenses AgreementZFN Use Licenses Agreement

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.