ECL DETECTION REAGENTS

Analysis Note

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Application

ECL^™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.Each system includes the following reagents, sufficient for labeling 5 to 10 µg nucleic acid and detecting 2000 to 4000 cm² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL^™ Detection Reagents, and ECL^™ Gold Hybridization Buffer.

General description

ECL^™ Detection Reagents.

Legal Information

ECL is a trademark of Cytiva