ECL DIRECT NUCLEIC ACID

Analysis Note

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Application

ECL^™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.Each system includes the following reagents, sufficient for labeling 5 to 10 µg nucleic acid and detecting 2000 to 4000 cm² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL^™ Detection Reagents, and ECL^™ Gold Hybridization Buffer.

Features and Benefits

Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL^™ Direct, Hybond^® N+, and Hyperfilm ECL. Eliminates handling, waste, and regulatory issues associated with the use of radioactivity. No need to strip blots before reprobing. For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses. Consistent results combining strong signals with very Low backgrounds.

General description

ECL^™ Direct Labeling and Detection System.

Legal Information

Hybond is a registered trademark of Cytiva

ECL is a trademark of Cytiva