Gamborgs B-5 Basal Medium with Minimal Organics, fine powder, plant cell culture tested

Application

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:as a component of liquid media for the tissue culture of Brassica rapa as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings as a component of solid GM medium for germination of Arabidopsis seeds

Formula variant

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).Media Formulation

General description

Gamborg’; B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg’;s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg’;s B-5 medium has been used for the culture of Annona squamosa callus.

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.

Quantity

Formulated to contain 3.2 grams of powder per liter of medium.