Application

The Dendritic Cell (DC) Generation Medium has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes. DC Generation Medium allows for the generation of DC from cryopreserved as well as freshly isolated cells. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed. The medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium. Recommended for:Human Monocytes (hMo), freshly isolated and cryopreservedHuman Mononuclear Cells (hMNC), freshly isolated

General description

DC Generation Medium (Ready-to-use)

Quality

All lots of PromoCell DC Media are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell DC Media is tested for the ability to support DC maturation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Keep the Cytokine Pack at -20°C for long-term storage or at 4 to 8°C for up to 2 weeks. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

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