Application

Research CategorySignaling

The unconjugated antibody (Cat. No. ABS233) is also available for immunoprecipitation and Western blotting applications.

Anti-PI3 Kinase p85, N-SH2 domain, Alexa Fluor^® 488 Conjugate, Cat. No. ABS233-AF488, detects PI3 Kinase p85 and is validated for use in immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: Q63787; also known as PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha) is encoded by the Pik3r1 (Gene ID 25513) gene in rat. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. It binds to activated (phosphorylated) protein tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 Kinase catalyzes the phosphorylation of PI(4,5)P2 (PIP2) inositol ring at the 3 position to generate PI(3,4,5)P3 (PIP3), a potent second messenger that mediates the action of several growth factors. PI3 kinase is essential for the insulin signaling and also plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), bringing p85-associated p110 proximal to its lipid substrate on the membrane. PI3 Kinase is also activated by Ras and by the beta and gamma subunits of heterotrimeric G-proteins.

Immunogen

GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody Alexa Fluor^® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Quality

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PI3 kinase p85 subunit in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HepG2 cells.

Specificity

This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787).