Anti-SELK

Analysis Note

ControlA20 cell lysate

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Anti-SELK Antibody is a highly specific rabbit polyclonal antibody, that targets Selenoprotein K & has been tested in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Selenoprotein K (SELK) is a selenoprotein that is localized at the endoplasmic reticulum, and highly expressed in the heart. SELK functions as an antioxidant that can bind to proteins such as the ER-associated degradation (ERAD) components p97 ATPase, Derlin-1 and -2, and SelS as well as proteins in the oligosaccharyltransferase complex. SELK may be involved in the Derlin-dependent ERAD of glycosylated misfolded proteins, is thought to be important in Ca2+ flux in immune cells, and is cleaved by m-calpain in resting macrophages.

Immunogen

KLH-conjugated linear peptide corresponding to human SELK.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal PBS with up to 0.1% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SELK in 15 µg of A20 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~10 kDa observed. Uncharacterized bands may appear in some lysate(s).